mutant mouse lines for nek1  (Jackson Laboratory)

Journal: Genes

Article Title: NEK1 Facilitates Cohesin Removal during Mammalian Spermatogenesis

doi: 10.3390/genes2010260

Figure Lengend Snippet: Nek1 kat2J/kat2J mice show decreased testes size. ( A ) Photograph of both Nek1 kat2J/kat2J and wild type littermate testis; ( B ) Wild type (black) and mutant Nek1 kat2J/kat2J (gray) testes and heart sizes, shown as a percentage of total body weights.

Article Snippet: NEK1 is highly expressed in murine germ cells, predominantly in the testes in spermatogonial cells and in prophase I stage spermatocytes [ , ] and at least two distinct mutant mouse lines for Nek1 , generated at the Jackson laboratories, display severely impaired fertility [ – ], indicating a probable involvement in meiotic progression.

Techniques: Mutagenesis

Journal: Genes

Article Title: NEK1 Facilitates Cohesin Removal during Mammalian Spermatogenesis

doi: 10.3390/genes2010260

Figure Lengend Snippet: 3 week old Nek1 kat2J/kat2J mice show disorganized testes morphology. 3 week old wild type ( A , C ) and Nek1 kat2J/kat2J testes ( B , D - F ) were stained with H&E ( A , B , E ) or TRA-98 antibody ( C , D , F ). Empty tubules are shown by the asterisks and empty tubules corresponding to those with no germ cell staining by the arrows.

Article Snippet: NEK1 is highly expressed in murine germ cells, predominantly in the testes in spermatogonial cells and in prophase I stage spermatocytes [ , ] and at least two distinct mutant mouse lines for Nek1 , generated at the Jackson laboratories, display severely impaired fertility [ – ], indicating a probable involvement in meiotic progression.

Techniques: Staining

Journal: Genes

Article Title: NEK1 Facilitates Cohesin Removal during Mammalian Spermatogenesis

doi: 10.3390/genes2010260

Figure Lengend Snippet: 8 week old Nek1 kat2J/kat2J mice show disorganized testes morphology and an increase in apoptotic cells. 8 week old wild type ( A , C , E ) and Nek1 kat2J/kat2J ( B , D , F ) testes were stained with H&E ( A , B ), TRA-98 ( C , D ) or TUNEL ( E , F ). The sperm tails in the wild type seminiferous tubules are shown with an asterisk, and the TUNEL positive cells in metaphase by the arrows and in the insets.

Article Snippet: NEK1 is highly expressed in murine germ cells, predominantly in the testes in spermatogonial cells and in prophase I stage spermatocytes [ , ] and at least two distinct mutant mouse lines for Nek1 , generated at the Jackson laboratories, display severely impaired fertility [ – ], indicating a probable involvement in meiotic progression.

Techniques: Staining, TUNEL Assay

Journal: Genes

Article Title: NEK1 Facilitates Cohesin Removal during Mammalian Spermatogenesis

doi: 10.3390/genes2010260

Figure Lengend Snippet: Nek1 kat2J/kat2J spermatocytes undergo normal synapsis. Wild type (not shown) and Nek1 kat2J/kat2J testes were stained with a variety of antibodies against meiotic prophase I proteins. Shown here is staining with SYCP3 (red), either SYCP1 or SYCP2 (green) and DAPI (blue) during the first four stages of prophase I; leptonema, zygonema, pachynema and diplonema.

Article Snippet: NEK1 is highly expressed in murine germ cells, predominantly in the testes in spermatogonial cells and in prophase I stage spermatocytes [ , ] and at least two distinct mutant mouse lines for Nek1 , generated at the Jackson laboratories, display severely impaired fertility [ – ], indicating a probable involvement in meiotic progression.

Techniques: Staining

Journal: Genes

Article Title: NEK1 Facilitates Cohesin Removal during Mammalian Spermatogenesis

doi: 10.3390/genes2010260

Figure Lengend Snippet: Nek1 kat2J/kat2J spermatocytes undergo normal double-strand break (DSB) initiation and repair. Wild type (not shown) and Nek1 kat2J/kat2J testes were stained with a variety of antibodies against meiotic prophase I proteins. Shown here is staining with SYCP3 (green), and either RAD51 or MHSH4 (red) during the zygotene and pachytene stages of prophase I.

Article Snippet: NEK1 is highly expressed in murine germ cells, predominantly in the testes in spermatogonial cells and in prophase I stage spermatocytes [ , ] and at least two distinct mutant mouse lines for Nek1 , generated at the Jackson laboratories, display severely impaired fertility [ – ], indicating a probable involvement in meiotic progression.

Techniques: Staining

Journal: Genes

Article Title: NEK1 Facilitates Cohesin Removal during Mammalian Spermatogenesis

doi: 10.3390/genes2010260

Figure Lengend Snippet: NEK1 protein acts later than FKBP6 in prophase I cells. ( A ) Wild type and Nek1 kat2J/kat2J testes sections were stained with an antibody to FKBP6 (brown stain) and counter stained with Hematoxylin; ( B ) Wild type and Nek1 kat2J/kat2J pachytene spermatocytes stained with anti-SYCP3 (red), anti-FKBP6 (green) and DAPI (blue).

Article Snippet: NEK1 is highly expressed in murine germ cells, predominantly in the testes in spermatogonial cells and in prophase I stage spermatocytes [ , ] and at least two distinct mutant mouse lines for Nek1 , generated at the Jackson laboratories, display severely impaired fertility [ – ], indicating a probable involvement in meiotic progression.

Techniques: Staining

Journal: Genes

Article Title: NEK1 Facilitates Cohesin Removal during Mammalian Spermatogenesis

doi: 10.3390/genes2010260

Figure Lengend Snippet: Cohesin protein SMC3 is not removed in the proper temporal manner in Nek1 kat2J/kat2J spermatocytes. Wild type (A–D) and Nek1 kat2J/kat2J (E–H) spermatocytes were stained with anti-SYCP3 (green), anti-SMC3 (red) and CREST autoimmune serum (blue). Cells in leptonema (A, E), zygonema (B, F), pachynema (C, G) and diplonema (D, H) were assessed for SMC3 localization (gray panels show SMC3 staining alone). The persistent XY chromosome staining of SMC3 in wild type cells is shown by the arrow. Wild type (I) and Fkbp6 −/− (J) spermatocytes were also stained with anti-SYCP3 (green), anti-SMC3 (red) and CREST autoimmune serum (blue), however only the diplotene stage is shown here.

Article Snippet: NEK1 is highly expressed in murine germ cells, predominantly in the testes in spermatogonial cells and in prophase I stage spermatocytes [ , ] and at least two distinct mutant mouse lines for Nek1 , generated at the Jackson laboratories, display severely impaired fertility [ – ], indicating a probable involvement in meiotic progression.

Techniques: Staining

Journal: Genes

Article Title: NEK1 Facilitates Cohesin Removal during Mammalian Spermatogenesis

doi: 10.3390/genes2010260

Figure Lengend Snippet: REC8 and STAG3 cohesin localization in Nek1 kat2J/kat2J spermatocytes. Nek1 kat2J/kat2J prophase I spermatocytes were stained with anti-SYCP3 (green) and either anti-REC8 (A, B), or anti-STAG3 (C, D) (red or gray).

Article Snippet: NEK1 is highly expressed in murine germ cells, predominantly in the testes in spermatogonial cells and in prophase I stage spermatocytes [ , ] and at least two distinct mutant mouse lines for Nek1 , generated at the Jackson laboratories, display severely impaired fertility [ – ], indicating a probable involvement in meiotic progression.

Techniques: Staining

Journal: Genes

Article Title: NEK1 Facilitates Cohesin Removal during Mammalian Spermatogenesis

doi: 10.3390/genes2010260

Figure Lengend Snippet: Chromosomes fail to align on the metaphase spindle correctly in Nek1 kat2J/kat2J spermatocytes. Nek1 kat2J/kat2J and wild type testis sections were stained with H&E, and cells undergoing metaphase I imaged. Wild type cells generally show proper alignment of chromosomes on the spindle (left), whereas the majority of cells in Nek1 kat2J/kat2J testis look abnormally aligned or have lagging chromosomes (arrow).

Article Snippet: NEK1 is highly expressed in murine germ cells, predominantly in the testes in spermatogonial cells and in prophase I stage spermatocytes [ , ] and at least two distinct mutant mouse lines for Nek1 , generated at the Jackson laboratories, display severely impaired fertility [ – ], indicating a probable involvement in meiotic progression.

Techniques: Staining